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scott gradia plic his mbp tev tbrucei h1 1  (Addgene inc)


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    Structured Review

    Addgene inc scott gradia plic his mbp tev tbrucei h1 1
    Scott Gradia Plic His Mbp Tev Tbrucei H1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+his6+mbp+vector/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/pm41709455-917-215-213
    Average 94 stars, based on 99 article reviews
    scott gradia plic his mbp tev tbrucei h1 1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9 C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described with a few variations. ..

    Article Title: Loss of age-associated increase in m 6 A-modified RNA contributes to GABAergic dysregulation in Alzheimer’s disease
    Article Snippet: After elution with 40 μL RNase-free water, RNA was quantified and assessed for quality on the Agilent TapeStation 4200 using the RNA ScreenTape assay and for purity on the Nanodrop. .. DART enzyme sequences (pCMV-APOBEC1-YTH [Addgene, #131636] and pCMV-APOBEC1-YTH mut [Addgene, #131637]) were cloned into the pET-His6-MBP vector (Addgene, #29656, a gift from Kate Meyer). .. A glycerol stab of Rosetta TM 2 (DE3) pLysS Singles TM Competent Cells (Sigma Aldrich, #71402) was transformed with DART-enzyme expression vectors and grown in Terrific Broth (Thermo Fisher, #22711022, supplemented with 50 μg/mL Kanamycin) overnight at 37 °C with shaking at 200 rpm.

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described [ ] with a few variations. ..

    Cloning:

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9 C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described with a few variations. ..

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described [ ] with a few variations. ..

    Plasmid Preparation:

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9 C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described with a few variations. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end.G0S2 fusionproteinwasproduced inBL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end. .. G0S2 fusion protein was produced in BL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol.

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end. .. G0S2 fusion protein was produced in BL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol.

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described [ ] with a few variations. ..

    Purification:

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9 C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described with a few variations. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end.G0S2 fusionproteinwasproduced inBL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end. .. G0S2 fusion protein was produced in BL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol.

    Article Title: RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
    Article Snippet: .. NSUN6 was cloned in a pET-His6-MBP vector (pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C) was a gift from Scott Gradia; Addgene plasmid #48286; http://n2t.net/addgene:48286 ; RRID:Addgene_48286) and purified as previously described [ ] with a few variations. ..

    Polymerase Chain Reaction:

    Article Title: Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia
    Article Snippet: .. The human HIG2 or HIG2 7–11 was subcloned by standard PCR into pET His6 MBP vector (addgene, #29708) producing fusion protein with a His6-MBP tag at the Nterminal end. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end.G0S2 fusionproteinwasproduced inBL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol. ..

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end. .. G0S2 fusion protein was produced in BL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol.

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end. .. G0S2 fusion protein was produced in BL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol.

    Sonication:

    Article Title: Identification of an intrinsic lysophosphatidic acid acyltransferase activity in the lipolytic inhibitor G 0 /G 1 switch gene 2 (G0S2)
    Article Snippet: .. Production and purification of bacterially expressed G0S2 The full-length mouse G0S2 cDNA was subcloned by standard PCR into pET His6 MBP vector (Plasmid 29708; Addgene) producing a G0S2 fusion protein with a His6-MBP tag at the N-terminal end.G0S2 fusionproteinwasproduced inBL21(DE3) Escherichia coli (Agilent Technologies, Santa Clara, CA, USA) with induction by addition of 0.5 mM IPTG (MilliporeSigma) at an A600 of 0.4–0.6 at 15°C and harvested after culturing for an additional 16 h. The cells were lysed by sonication-collagenase; the expressed G0S2 fusion protein was purified using nickel nitrilotriacetic acid (NTA) agrose beads according to the commercial protocol. ..



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